Figure 3.
The combination of GCK silencing and iberdomide shows enhanced anti-MM effects. (A) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 48 hours, then treated with different doses of Iberdomide for 72 hours. Cell proliferation was detected using MTS. (B) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 48 hours to induce GCK knockdown (shGCK), then treated with 1 nM iberdomide for 24 hours. The protein expression level of GCK, c-MYC, and IKZF1 were detected by western blot using β-actin as a loading CT. (C-D) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 2 days to induce GCK knockdown (shGCK), then treated with 1 nM iberdomide for 5 days. Cell apoptosis was detected by flow cytometry after Annexin V and 7-AAD staining (C); cell cycle was analyzed by flow cytometry after PI staining (D). ∗∗P < .01 (by 1-way ANOVA).

The combination of GCK silencing and iberdomide shows enhanced anti-MM effects. (A) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 48 hours, then treated with different doses of Iberdomide for 72 hours. Cell proliferation was detected using MTS. (B) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 48 hours to induce GCK knockdown (shGCK), then treated with 1 nM iberdomide for 24 hours. The protein expression level of GCK, c-MYC, and IKZF1 were detected by western blot using β-actin as a loading CT. (C-D) DOX-inducible shGCK MM.1S cells were treated with 400 ng/mL DOX for 2 days to induce GCK knockdown (shGCK), then treated with 1 nM iberdomide for 5 days. Cell apoptosis was detected by flow cytometry after Annexin V and 7-AAD staining (C); cell cycle was analyzed by flow cytometry after PI staining (D). ∗∗P < .01 (by 1-way ANOVA).

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