Genome-wide CRISPR screens identify Chr 7 CYCLOPS genes as new targets for therapy. (A) Workflow showing the identification of Chr 7 essential and CYCLOPS genes using 23 DepMap (green text) and 22 independently published screens (blue text) in AML cell lines. (B) Venn diagram showing overlap of essential genes between the 2 methods of in silico data mining for Chr 7 essential genes. Pearson χ2 test was used to calculate the statistical significance between overlaps. (C) Volcano plot showing Pearson correlations between CRISPR dependency (DepMap) and mRNA expression (CCLE) for Chr 7 essential genes. The genes whose expression levels were significantly correlated (adjusted P < .05) with either increased or decreased sensitivity to CRISPR-Cas9 knockout were categorized as ARGuS genes (red) and CYCLOPS genes (blue), respectively. Chr, chromosome.
