Overall clustering and description of scRNA-seq results of PBMCs from patients with hemophilia with inhibitors. (A) In total, 19 subsets were clustered and visualized through uniform manifold approximation and projection (UMAP). (B) The dot plot showed the expression levels of the markers for the annotation of the 19 subsets. (C) The cell ratios of each subset in each sample. The legend in panel A also applies to panel C. (D-H) Student t test was used to assess the difference of main lymphocyte clusters’ ratios in the total lymphocytes between the HA and HB groups, including T cells (D), B cells (E), NK cells (F), plasma cells (G), and proliferative cells (H). DNT, double-negative T; mDCs, myeloid DCs.