DNA methylation profiles. (A) Hierarchical clustering analysis of DNA methylation profiles on 23 pretreatment samples (left). Twenty thousand CpG probe blocks for the whole genome were randomly selected. Hierarchical clustering analysis of promoter methylation profiles of 119 gene blocks for 113 ISGs (right). Pretreatment samples were used and methylation (β values) of gene blocks in CpG islands in the region from TSS to −200 bp upstream was analyzed. Seventeen genes were hypomethylated in pts who failed to respond or relapsed after VEN/DEC treatment (R/R pts) compared to pts who achieved CR/CRi (CR pts). (B) Methylation levels (β values) of PPARG in CpG islands within regions from the TSS to −200 bp upstream were compared between R/R and CR pts. (C) Methylation levels (β values) of PPARG in CpG islands within regions from the TSS to −1500 bp upstream were compared between the pretreatment and posttreatment samples obtained from CR and R/R pts. Differences between the groups were tested using the 2-tailed Student t test. ∗P < .05.