Analysis of splenic PCs shortly after immunization. (A) Example of gating on PCs in AID-TOM (upper) and REL-AID (lower) mice from the mid-term cohort (also refer to Figure 5A). Red colored cells are TOM⁺ B cells only and green colored cells are TOM⁺YFP⁺ B cells (same gating of the first 2 cytogram in Figure 5B). (B) Percentages of PCs among total TOM⁺ B cells in AID-TOM and REL-AID mice (upper) and percentages of YFP⁺ among TOM⁺ B cells in the total TOM⁺ and in the PC fraction in REL-AID mice (each line connects the points for 1 mouse) (lower). Mean and standard error of the mean are shown by a red and 2 black lines, respectively. The P values of the Mann-Whitney (upper) and paired Wilcoxon (lower) test are shown. ns, nonsignificant.