Relationship between the levels of TOM⁺ and TOM⁺/YFP⁺ B cells in secondary lymphoid organs and in the BM. (A-B) Schematic representation of the induction and immunization protocol for analysis either mid-term (A) or long-term (B) cohorts. (C-D) Analysis of TOM⁺ and TOM⁺/YFP⁺ B cells in secondary lymphoid organs for the mid-term (C) and long-term (D) cohorts for AID-TOM control (left) and REL-AID mice (right) in the SP, LN, BM, and in the IP. Top: percentage of TOM⁺ B cells among total live B cells (CD19⁺ B220^+/−). Bottom: percentage of TOM⁺/YFP⁺ B cells among TOM⁺ B cells. In panel C, the case with a diffuse aggressive B-cell lymphoma is indicated by an arrow. Mean and SEM are shown by a red and 2 black lines, respectively. The P value of a 2-way ANOVA for REL effect is shown on the top of the graph. Each experiment has been done at least 4 times. BM, bone marrow; LN, lymph nodes; IP, peritoneum.