Relationships between the kinetics of PB TOM⁺ and TOM⁺/YFP⁺ with CD80 expression. (A) Box plot of the mean percentage (± standard error of the mean) of PB TOM⁺ cells among live B cells (CD19⁺ B220^+/−) in AID-TOM and REL-AID mice over time post-tamoxifen induction. The dashed red line indicates the end of immunization boost. The P value of a 2-way analysis of variance (ANOVA) for REL effect is shown. (B) Relationship between the percentage of TOM⁺/YFP⁺ among total TOM⁺ B cells (x-axis) and that of TOM⁺ B cells among total B220⁺ B cells (y-axis) in REL-AID mice at month 2 (top graph) and month 12 (bottom graph). The correlation curve is shown in red. The values of the Pearson correlation coefficient r and of its P values are given in each graph. (C) Distribution of the percentage of TOM⁺/YFP⁺ B cells among total TOM⁺ B cells over time in REL-AID mice. The red dot represents the mouse with the highest percentage and green dot the mouse with the lowest percentage. The dashed red line indicates the end of immunization boost. Mean, standard error of the mean, and number n of mice are indicated above the graph. The P value of the 1-way ANOVA for time effect is shown. (D) CD80 expression levels in TOM⁺/YFP^– B cells from either AID-TOM and REL-AID mice as well as TOM⁺/YFP⁺ B cells from REL-AID mouse model. Mean and SEM are shown for each B-cell sample by a red and 2 black lines, respectively. Mann Whitney test P values are shown. Each experiment has been done at least 4 times. MFI, mean fluorescence intensity.