Loss of enhancer and promoter activity in CREBBP KAT-PM cells. (A) Heat maps of regions with significant H3K27Ac loss in RL cells with CREBBP KAT-PM (R1446C, Y1482N, Y1503C) compared with WT (FDR <0.05), also showing H3K27Ac abundance in CREBBP KO cells. (B-C) Density plots (B) and violin plots (C) of relative change compared with WT for histone posttranslational modifications and chromatin accessibility in RL cells with CREBBP KO different KAT-PMs (R1446C, Y1482N, Y1503C), showing regions from panel A. (D) Relative abundance of H3K27Ac in primary FL tumor cells, normalized to the average of CREBBP WT tumors, showing regions of consensus H3K27Ac loss in CRISPR edited CREBBP KAT-PM RL and HT cells compared with isogenic WT controls. (E) Density plot of relative H3K27me3 in RL CREBBP KAT-PM (R1446C, Y1482N, Y1503C) or KO CRISPR edited cells compared with isogenic WT controls, showing regions from panel A.