Figure 4.
Proliferation and expansion of Vδ2+ T cells in response to graded TCR stimulation. (A) Proliferation and expansion statistics for experiments found in (B). Number of proliferating Vδ2+ cells corresponds to the integral under the curve, highly proliferating cells refers to proliferating Vδ2+ cells with CTV intensity ≤103. The fold expansion of Vδ2+ T cells was calculated as follows: ([Vδ2+ T cells per CD3+ T cells in PBMCs [%] after stimulation])/(Vδ2+ T cells per CD3+ T cells in PBMCs [%] before stimulation). (B) CTV FACS plots of Vδ2+ T cells from HH (n = 3) and HDs (n = 3) after stimulation with ZOL (Vδ2Vγ9-specific stimulation), IMMU510 (pan γδ stimulation), and αCD3/αCD28 (pan T-cell stimulation) in PBMCs ∗P < .05; ∗∗P < .01;. ns, not significant.