Figure 3.
DFO does not increase the yield of HH Vδ2+ T cells, and HD Vδ2+ cells proliferate vigorously when added to allogeneic TCRαβ-depleted HH PBMCs. (A) 100 μM DFO completely blocks proliferation of Vδ2+ T cells in ZOL-stimulated HD PBMCs (n = 7). (B) Dose titration of DFO to identify a physiological window for T-cell proliferation (n = 1). (C) DFO does not improve the proliferation and yield of Vδ2+ T cells from patients with HH in ZOL-stimulated cultures (n = 3). Compared with ZOL-stimulated PBMCs from HD (n = 2), Vδ2+ T-cell proliferation from individuals with HH (n = 3) is negatively and dose dependently affected at even lower concentrations of DFO than their counterparts from healthy individuals. (D) HH Vδ2+ T cells (n = 3) proliferate when stimulated with ZOL in irradiated PBMCs from HDs, but the increase in number is significantly less than that found in HD Vδ2+ T cells (n = 3), which reveal normal, strong proliferation when stimulated in irradiated PBMCs from patients with HH. Overlay: Superimposed CTV histogram for HD Vδ2+ T cells responding in HH PBMCs (blue) vs HH Vδ2+ T cells responding in autologous PBMCs (red). ∗∗∗∗P < .0001; ns, not significant.

DFO does not increase the yield of HH Vδ2+ T cells, and HD Vδ2+ cells proliferate vigorously when added to allogeneic TCRαβ-depleted HH PBMCs. (A) 100 μM DFO completely blocks proliferation of Vδ2+ T cells in ZOL-stimulated HD PBMCs (n = 7). (B) Dose titration of DFO to identify a physiological window for T-cell proliferation (n = 1). (C) DFO does not improve the proliferation and yield of Vδ2+ T cells from patients with HH in ZOL-stimulated cultures (n = 3). Compared with ZOL-stimulated PBMCs from HD (n = 2), Vδ2+ T-cell proliferation from individuals with HH (n = 3) is negatively and dose dependently affected at even lower concentrations of DFO than their counterparts from healthy individuals. (D) HH Vδ2+ T cells (n = 3) proliferate when stimulated with ZOL in irradiated PBMCs from HDs, but the increase in number is significantly less than that found in HD Vδ2+ T cells (n = 3), which reveal normal, strong proliferation when stimulated in irradiated PBMCs from patients with HH. Overlay: Superimposed CTV histogram for HD Vδ2+ T cells responding in HH PBMCs (blue) vs HH Vδ2+ T cells responding in autologous PBMCs (red). ∗∗∗∗P < .0001; ns, not significant.

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