Vδ2⁺ T-cell numbers do not increase in PBMCs of patients with HH in response to ZOL stimulation. (A) The Vδ2⁺ T-cell pool does not expand after ZOL stimulation in PBMCs of patients with chronic iron overload (C282Y n = 5, compound n = 5) compared with PBMCs of HD (n = 18). Revealed are percentages of Vδ2⁺ T cells per CD3⁺ before and after ZOL stimulation (day 7). Vδ2⁺ proportion/CD3⁺ is significantly increased on day 7 compared with day 0 in HD PBMCs (analysis of variance, P < .0001) but not in PBMCS of individuals with HFE (P = .6582). (B) Fold-expansion rate of Vδ2⁺ subset is significantly different in HD (n = 10) and HH (n = 5 C292Y, n = 5 compound) (P = .093). ∗∗P < .01; ∗∗∗∗P < .0001; ns, not significant.