Differential CGD genotype–driven host immune responses at the intestinal barrier. Multicolor flow cytometry analysis of WT, gp91^phox–/–, and p47^phox–/– mice from IRCM before and after DSS treatment showing IgA, IgM, or IgG-coated bacteria (SYBR⁺) from mouse fecal pellets and fold change relative to WT (bar graph) (A); colon LP frequencies of IgA⁺ and IgG⁺ ASCs (CD138⁺) (B); Treg (FoxP3⁺), Th17 (RORγT⁺), and Th1 (T-bet⁺) CD4⁺ T cells (C); and frequencies of IL-10⁺, IL-1β⁺, myeloid cells (CD11b⁺, TCRb^–), macrophages (F4/80⁺), DCs (MHCII⁺, CD11c⁺), monocytes (Ly6C⁺), and neutrophils (Ly6G⁺) (D). (E) Measurements of IFN-γ, TNF-ɑ, IL-2, and IL-10 levels in sera from WT, gp91^phox–/–, and p47^phox–/– mice from IRCM at baseline and after DSS treatment. The data are presented as means ± standard error of the means. All baseline data are from 1 experiment (n = 3-4 mice per group) and all DSS data are representative of 2 independent experiments (n = 3-4 mice per group). Significance was determined using 1-way and 2-way ANOVA with Holm-Šídák multiple comparison test (∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001). Freq, frequency; TNF, tumor necrosis factor; Treg, regulatory T cell.