Gata2 associated phenotypes are accompanied by proliferation defects and genomic instability. (A) PCA of E14, (B) adult, and (C) aged WT (red) and Gata2^+/− (blue) HSCs. Each dot represents the transcriptome of an individual sample. (D) Hallmark GSEA of Myc targets in E14, (E) adult, and (F) aged WT and Gata2^+/− HSCs. (G) Commonly upregulated genes in Myc Targets V1 GSEA upregulated in all conditions in Gata2^+/− HSCs. (H) Proliferation analysis of adult (I) and aged WT and Gata2^fl/+ HSCs. (J) Proliferation analysis of young and (K) aged WT and vav-Cre;Gata2^fl/+ mice 3 days after carboxyfluorescein diacetate succinimidyl ester (CFSE) staining. (L) Quantification of γH2AX signals in aged-WT and aged-Gata2^+/− after transplantation of LK, LSK, HSC, MPP, HPC1, and HPC2 compartments of the BM. (M-N) representative image of a WT and GATA2^+/− K562 cell during anaphase of cell cycle with (M) indicating a normal proliferation event and (N) indicating an aberrant proliferation event indicated by the arrowhead and quantified in (O) N = 3 biological replicates, in total 50 anaphases scored for WT and 49 anaphases scored for GATA2^+/−. ∗P < .05; ∗∗P < .01; div, cell division; MFI, mean fluorescence intensity; PC, principal component.