Nilotinib reduces brain and lung hemorrhages in late-stage treatment intervention. (A) Schematic overview of the histology study conducted on day 8 after infection. (B) Parasitemia in mice on day 7 and 8 after infection (n = 6 mice per group). (C) Representative hematoxylin and eosin (H&E)–stained brain sections of uninfected (Un), infected (Inf), and nilotinib (Nil)-treated mice. Hemorrhage (white dashed box) in the cerebral cortex area on day 8 after infection (magnification ×10; scale bar = 100 μm; magnification ∼×3.6, boxed inset). (D) Total brain hemorrhage score. (E) Individual brain area hemorrhage score. (F) Representative H&E lung sections (magnification ×20; scale bar = 50 μm) showing hemorrhage area (black dashed box; magnification ∼×4, boxed inset). Lungs were collected on day 8 after infection. (G) Lung hemorrhage score. (H) Lung congestion score. (I) Correlation of parasitemia and brain hemorrhage score. (J) Correlation of parasitemia and lung hemorrhage score. A 1-way ANOVA, followed by Dunnett multiple comparisons test (compared with the infected group) was used to analyze the data. ∗P < .05; ∗∗P < .01; ∗∗∗P < .01.