Figure 3.
Liver-derived TG2 cross-links fibrinogen ex vivo. (A-C) Liver and plasma were isolated from naïve wild-type and TG2−/− mice. (A) Digital capillary rendering of western blot illustrating robust detection of TG2 in liver homogenates from wild-type mice, but not TG2−/− mice. (B) Plasma FXIII-A levels were quantified by capillary western blotting (Wes instrument) and expressed as peak area. (C) Plasma fibrinogen concentration was measured by ELISA. Results from individual mice are plotted and bars represent mean ± SEM. (D-E) Liver homogenates (D, 1:3 dilution; E, 1:9 dilution) generated in the presence of bivalirudin and tranexamic acid were incubated with human fibrinogen (10 μg/mL) for 10 minutes and levels of cross-linked fibrin(ogen) determined using anti-Fibα or anti-Fibγ antibodies by capillary western blotting. Representative digital capillary renderings are shown.

Liver-derived TG2 cross-links fibrinogen ex vivo. (A-C) Liver and plasma were isolated from naïve wild-type and TG2−/− mice. (A) Digital capillary rendering of western blot illustrating robust detection of TG2 in liver homogenates from wild-type mice, but not TG2−/− mice. (B) Plasma FXIII-A levels were quantified by capillary western blotting (Wes instrument) and expressed as peak area. (C) Plasma fibrinogen concentration was measured by ELISA. Results from individual mice are plotted and bars represent mean ± SEM. (D-E) Liver homogenates (D, 1:3 dilution; E, 1:9 dilution) generated in the presence of bivalirudin and tranexamic acid were incubated with human fibrinogen (10 μg/mL) for 10 minutes and levels of cross-linked fibrin(ogen) determined using anti-Fibα or anti-Fibγ antibodies by capillary western blotting. Representative digital capillary renderings are shown.

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