Case 2 variant RH alleles and Rh proteins. (A) Schematic of the single-nucleotide variant lines associated with RHD∗DIIIa, RHD∗DAU0, and RHCE∗ce^S. Exons 1 and 10 are labeled for each allele. The numbers below the exon boxes indicate the nucleotide changes. (B) The amino acid differences for the corresponding variant antigens are indicated by green circles. The amino acid changes resulting in V and VS antigen expression are indicated. The specific amino acids responsible for hr^B and hr^S expression are not known. The predicted Rh phenotype reflects Rh antigen expression from all 4 RH alleles. Figure created with BioRender.com.