Activation of Nrf2 promotes differentiation. (A-B) Gene set enrichment analysis³⁹ of control and Nrf2^–/– SEDM day 3 RNA-seq data. (A) Analysis of gene sets involved in erythrocyte development, homeostasis, and heme and (B) inflammatory pathways. (C-D) SEPs were harvested from WT or Irg1^–/– SEDM cultures treated with vehicle, 125-μM OI, or 30-μM DMF for 3 days. (C) Analysis of mRNA expression of erythroid-specific genes by qRT-PCR. (D) Analysis of stress BFU-E by colony assay (n = 4 per group; 1-way ANOVA/Tukey test).