Bioactive lipid mediators in CSP and RSP storage bags. Data were mean-centered and divided by the standard deviation of each variable to normalize. (A) Heat map with individual lipid mediator levels at BL (fresh) and after 14 days of 4°C storage, and 7 days of RT storage. Color codes for individual donors in first row. The BL (fresh) data were averaged from 2 different collections. (B-C) Volcano plots of CSPs, plotted as logarithmic false discovery rate (FDR; Q = 1%, discoveries above the dotted line as blue circles) and logarithmic fold change (B) and 2-dimensional (2D) score plot of PC analysis of CSP (green triangles, baseline; blue squares, CSPs) (C). (D-E) Volcano plots of RSPs (discoveries shown above the dotted line as red circles) (D) and 2D score plot of PC analysis of RSPs (green triangles, baseline; red circles, RSPs) (E). n = 9 (only paired data). Statistical significance was assessed with a FDR of Q = 1% (89 comparisons). FC, fold change; RT, room temperature.