Fc modification enhances RBC clearance through ADCC. (A-B) ADCC assays were performed using monocyte-depleted PBMCs in the presence bromelain-treated (A) or untreated (B) RhD^pos RBCs opsonized with anti-RhD Abs. The release of Hb, indicating RBC lysis, was measured by enzyme-linked immunosorbent assay (ELISA). ADCC was calculated with subtraction of “no Ab” control background. All the mAbs were tested alongside RhD-pIgG, but each group of mAbs is presented in a separate figure to allow for a clear comparison. Data are the average of 2 to 4 replicates, and error bars denote SEM. Statistical analyses were conducted using 1-way ANOVA, followed by Dunnett multiple comparison test (GraphPad Prism v9). ∗P < .05; ∗∗P < .01.