Expression of CD234 and CD235a on erythrocytes in thalassemia carriers and healthy controls. Levels of CD234 and CD235a on red blood cells were determined using flow cytometry and expressed as MFI (A-B, E-F) and further normalized to area of cell surface (C-D, G-H). Comparison of levels of unnormalized (A) and normalized (C) levels of CD234 between healthy controls (N = 73) and thalassemia carriers (N = 83). Comparison of unnormalized (B) and normalized (D) levels of CD234 between healthy controls and subgroups of thalassemia carriers including α-silent carriers (n = 32), α-thalassemia minor (n = 35), β-thalassemia minor (n = 9), and β-thalassemia minor combined with α-silent or α-thalassemia minor (n = 7). Comparison of levels of unnormalized (E) and normalized (G) levels of CD235a between healthy controls and thalassemia carriers. Comparison of unnormalized (F) and normalized (H) levels of CD235a between healthy controls and subgroups of thalassemia carriers including α-silent carriers, α-thalassemia minor, β-thalassemia minor, and β-thalassemia minor combined with α-silent or α-thalassemia minor. Statistical significance was determined using unpaired Student t test (A,C,E,G) and 1-way analysis of variance (B,D,F,H). ∗P < .05; ∗∗∗P < .001; ∗∗∗∗P < .0001. ns, not significant.