Ferroptosis modulation shapes B-cell phenotype in Trsp KO model. (A) Schematic representation of feeding a Vit E–deficient diet. (B) Frequency of B220⁺, CD11b⁺Gr-1⁺, and CD11b⁺Gr-1^– cells in the BM of each group administered Vit E–deficient diet for 6 weeks (Trsp KO/Vit E–deficient diet group, n = 2 mice; other groups, n = 3 mice per group). (C) MFI of C11-BODIPY 581/591 and CellROX staining in B220⁺ BM cells of each group administered Vit E–deficient diet for 3 weeks. For C11-BODIPY 581/591, control/control diet group included 4 mice; other groups, 5 mice per group. For CellROX, control/control diet group included 3 mice; other groups, 4 mice per group. Representative histograms of C11-BODIPY 581/591 and CellROX staining in B220⁺ BM cells of each group are provided. (D) MFI of C11-BODIPY 581/591 and CellROX staining in pre–pro-B, pro-B, pre-B, and immature B cells in BM cells of each group (control/control diet group, n = 3 mice; other groups, n = 4 mice per group). (E) Schematic representation of feeding a Vit E–rich diet. (F) Frequency of B220⁺ CD11b⁺Gr-1⁺ and CD11b⁺Gr-1^– cells in the spleen of each group administered Vit E–rich diet for 6 weeks (n = 3 per group). P values were calculated by a 2-sided Student t test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. ns, not significant.