Figure 7.
SRGN–/– mice have decreased bone marrow stem cell populations. Multipotent progenitor population cell counts determined by flow cytometry of whole marrow cells from 52-week-old mice and selection as follows: MPP2: CD45+Lin–Sca-1+c-Kit+Flt3–CD150+CD48+ (A); MPP3: CD45+Lin–Sca-1+c-Kit+Flt3–CD150–CD48+ (B); MPP4: CD45+Lin–Sca-1+c-Kit+Flt3+ (C). Each point represents a single mouse (n = 9). (D-E) Short-term HSC (ST-HSC) (D) and long-term HSC (LT-HSC) counts (E) determined by flow cytometry of whole marrow cells from 52-week-old mice and selection as follows: LT-HSCs: CD45+Lin–Sca-1+c-Kit+Flt3–CD150+CD48–; ST-HSCs: CD45+Lin–Sca-1+c-Kit+Flt3–CD150–CD48+. Each point represents a single mouse (n = 9). Significance: ns is indicated by P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. All error bars represent the SEM.

SRGN–/– mice have decreased bone marrow stem cell populations. Multipotent progenitor population cell counts determined by flow cytometry of whole marrow cells from 52-week-old mice and selection as follows: MPP2: CD45+Lin–Sca-1+c-Kit+Flt3–CD150+CD48+ (A); MPP3: CD45+Lin–Sca-1+c-Kit+Flt3–CD150–CD48+ (B); MPP4: CD45+Lin–Sca-1+c-Kit+Flt3+ (C). Each point represents a single mouse (n = 9). (D-E) Short-term HSC (ST-HSC) (D) and long-term HSC (LT-HSC) counts (E) determined by flow cytometry of whole marrow cells from 52-week-old mice and selection as follows: LT-HSCs: CD45+Lin–Sca-1+c-Kit+Flt3–CD150+CD48–; ST-HSCs: CD45+Lin–Sca-1+c-Kit+Flt3–CD150–CD48+. Each point represents a single mouse (n = 9). Significance: ns is indicated by P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. All error bars represent the SEM.

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