RHOA deficiency prevents platelet clearance in vivo and in vitro. (A) Representative example of immunoblot analysis of RHOA expression after polyI:C treatment. Density quantification was performed using ImageJ software, and ratios were calculated after normalization of the RT-vehicle control. (B) In vivo platelet survival of cold-stored murine platelets in vitro either from wild-type (WT) or RHOA-deficient mice. Two independent experiments with 5 mice per group. (C) AUC of data depicted in panel B. (D) Macrophage-dependent in vitro phagocytosis assay using either WT or RhoA^Δ/Δ murine cold-stored platelets. Three independent experiments were performed in triplicate. ∗P < .05; ∗∗∗P < .001.