The adrenal stroma recruits and supports circulating hematopoietic progenitors. (A) Experimental design for EMH induction after transplant with GFP⁺ BM. (B-C) CFU assay, number of total colonies (left, black labels) and GFP⁺ colonies (right, green labels) from total per single adrenal gland (B) and 10 000 CD45⁺ total BM cells (C); n = 6 per experimental group, 2 independent experiments). (D) General experimental design for homing assay. (E) CD45⁺ cells in EMH-induced adrenals glands retrieved from mice treated with plerixafor (CXCR4i), evaluated by flow cytometry at 16 hours after treatment (2 independent experiments, n = 8 for control groups, and n = 10 for EMH-induced groups). (F) Total cells were recovered after 8-day CFU assay in complete methylcellulose and the percentage of GFP⁺ cells from the recovered cells is shown, as quantified by flow cytometry (n = 9 control mice, and n = 8 experimental mice). Data are represented as mean ± SD and groups were compared with 1-way ANOVA followed by Tukey multiple correction test (E) or 2-tailed, unpaired Student t test (F). ns, not significant.