Figure 2.
The adrenal gland supports functional, serially transplantable HSPCs. (A) Flow cytometry analysis of the BM, blood, and adrenal glands of control vs EMH-treated mice; representative panels gated within the CD45+ Lin− gate are shown (control, n = 10; EMH, n = 10 mice; 3 independent experiments). (B) Experimental design of the competitive transplant. The total content of CD45.2+ cells retrieved from 6 adrenal glands were transplanted into a lethally irradiated CD45.1 recipient together with 125 000 CD45.1.2 total BM cells from a competitor mouse. (C-D) Evolution of the CD45.2 donor engraftment in serial transplantation as measured in the peripheral blood and BM, respectively, by flow cytometry (control, n = 7; EMH, n = 8 mice; 2 independent experiments). Data are shown as mean ± standard error of the mean. Differences were assessed using a 2-tailed unpaired Student t test.

The adrenal gland supports functional, serially transplantable HSPCs. (A) Flow cytometry analysis of the BM, blood, and adrenal glands of control vs EMH-treated mice; representative panels gated within the CD45+ Lin− gate are shown (control, n = 10; EMH, n = 10 mice; 3 independent experiments). (B) Experimental design of the competitive transplant. The total content of CD45.2+ cells retrieved from 6 adrenal glands were transplanted into a lethally irradiated CD45.1 recipient together with 125 000 CD45.1.2 total BM cells from a competitor mouse. (C-D) Evolution of the CD45.2 donor engraftment in serial transplantation as measured in the peripheral blood and BM, respectively, by flow cytometry (control, n = 7; EMH, n = 8 mice; 2 independent experiments). Data are shown as mean ± standard error of the mean. Differences were assessed using a 2-tailed unpaired Student t test.

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