The adrenal gland can be hormonally induced to host hematopoietic cells. (A) General experimental design to induce EMH in the adrenal gland. (B) Macroscopic picture of freshly isolated adrenal glands. (C) Representative images of hematoxylin and eosin (H&E) stains for vehicle (top) and EMH-induced (bottom) adrenal glands. The boxed area is magnified on the right of each image. Scale bar for inset represents 30 μm. (D) Representative images of CD45 IHC stain in an adrenal gland. The boxed area is magnified on the right of each image. Scale bar for inset represents 30 μm. (E) Number of CD45⁺ cells per adrenal gland, measured by flow cytometry (control, n = 4; EMH, n = 6 mice; 2 independent experiments). (F) CFU assay, number of colonies per adrenal gland (control, n = 4; EMH, n = 6 mice; 2 independent experiments). (G) Number of hematopoietic colonies per adrenal gland obtained in a CFU assay from mice treated with the different components of the induction protocol (n = 4 for all groups). Data are represented as mean ± standard deviation (SD). Differences were assessed using unpaired, 2-tailed Student t test (E-F) or with 1-way analysis of variance (ANOVA) followed by Holms-Šidák multiple correction test (G). P values are indicated in the graphs.