Flowchart of data analyses. The gnomAD data set includes 807 162 subjects (1 614 324 alleles). Among this population, 6321 distinct ADAMTS13 variants were identified, of which 2525 variants were short insertions/deletions and single-nucleotide variants. We applied our pathogenicity criteria (supplemental Table 2) to identify pathogenic variants among the 2525 variants. Overall, 758 distinct variants were classified as pathogenic; among them, 418 variants (55%) were identified in only 1 individual (ie, were unique). Of these 758 pathogenic ADAMTS13 variants, 140 had been previously reported to cause hTTP and 618 were newly predicted to be pathogenic. A total of 10 154 alleles were carrying these 758 pathogenic variants, 7795 (77%) reported variants and 2359 (23%) predicted variants. Missense variants were the most common form of the pathogenic variants (61%), and they were responsible for the majority of the 10 154 affected alleles by pathogenic variants (92%). A start-lost variant is a point mutation in the ATG start codon, which prevents the original start translation site from being used. A stop-gained is a DNA variant that changes at least 1 base of a codon, leading to a premature stop codon.