Figure 2.
Effect of other mAbs on the binding of R21D10 to platelet αIIbβ3. (A) Washed platelets were preincubated with 40 μg/mL 10E5, 7E3, or unlabeled R21D10 for 15 minutes, then 10 μg/mL Alexa Fluor 488–labeled R21D10 was added for 15 minutes and detected by flow cytometry. (B-C) Washed platelets were preincubated with 40 μg/mL R21D10, unlabeled 10E5 (B), or unlabeled 7E3 (C) for 15 minutes, after which 10 μg/mL Alexa Flour 488–labeled 10E5 or 7E3 was added for 15 minutes and detected by flow cytometry. (D) T6-activated platelets were preincubated with R21D10 immunoglobulin G (IgG), R21D10 Fab, or unlabeled 7E3 for 15 minutes, and Alexa Fluor 488–labeled 7E3 binding was detected as above. (E-F) The effect of β3 PSI domain–specific mAb 7H2 on R21D10 binding (E) and the effect of R21D10 on 7H2 binding (F) to unactivated platelets was detected as above.

Effect of other mAbs on the binding of R21D10 to platelet αIIbβ3. (A) Washed platelets were preincubated with 40 μg/mL 10E5, 7E3, or unlabeled R21D10 for 15 minutes, then 10 μg/mL Alexa Fluor 488–labeled R21D10 was added for 15 minutes and detected by flow cytometry. (B-C) Washed platelets were preincubated with 40 μg/mL R21D10, unlabeled 10E5 (B), or unlabeled 7E3 (C) for 15 minutes, after which 10 μg/mL Alexa Flour 488–labeled 10E5 or 7E3 was added for 15 minutes and detected by flow cytometry. (D) T6-activated platelets were preincubated with R21D10 immunoglobulin G (IgG), R21D10 Fab, or unlabeled 7E3 for 15 minutes, and Alexa Fluor 488–labeled 7E3 binding was detected as above. (E-F) The effect of β3 PSI domain–specific mAb 7H2 on R21D10 binding (E) and the effect of R21D10 on 7H2 binding (F) to unactivated platelets was detected as above.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal