Prevalence and distribution of pregain deletions in HY patients with newly diagnosed myeloma. (A) Cumulative distribution plot of pregain deletions in 55 HY/IgH-neg patients with NDMM. Top of plot shows TSGs that were enriched more than the median number of patients per 1 Mb bin with a pregain deletion on the given chromosome. (B) Enlarged distribution of pregain deletions on chromosome 14 involving the TSG MAX. (C) Copy number plot showing pregain deletion, including MAX, in patient MM184. (D) RNAseq transcript levels of patients with NDMM from the German cohort with patient MM184 highlighted in yellow who showed a clear MAX downregulation. (E) Enlarged distribution of pregain deletions per 1 Mb bin on chromosome 10 around the oncogene NT5C2. (F) Copy number plot showing a pregain deletion near NT5C2 in patient MMRF_2532. (G) RNAseq transcript levels of patients with NDMM from MMRF with patient MMRF_2532 highlighted in yellow, showing a clear overexpression of NT5C2. In panels C, F, the black and orange horizontal lines represent the total and minor copy number, respectively. The black, red, green, and blue vertical lines represent translocations, deletions, duplications, and inversions. For translocations, the partner chromosome is reported on the top of the line.