Figure 7.
CORM-401 modulates the hemolysate-induced activation of pro-oxidation and proinflammatory pathways in the kidney of SS mice. Western blot analysis with specific antibodies against phosphorylated (p)-Nrf2 and Nrf2 in the kidney from AA and SS mice in steady state and exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 8% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA animals; #P < .05 compared with SB-treated animals by 1-way ANOVA. (B) Western blot analysis with specific antibodies against HO-1 and reduced NADP dehydrogenase (quinone)-1 (Nqo1) in the kidney from AA and SS mice in steady state and exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 11% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA animals; #P < .05 compared with SB-treated animals by the 1-way ANOVA. (C) Western blot analysis with specific antibodies against VCAM-1 and ICAM-1, in the kidney from AA and SS mice exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 11% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle treated animals by the 1-way ANOVA.

CORM-401 modulates the hemolysate-induced activation of pro-oxidation and proinflammatory pathways in the kidney of SS mice. Western blot analysis with specific antibodies against phosphorylated (p)-Nrf2 and Nrf2 in the kidney from AA and SS mice in steady state and exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 8% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA animals; #P < .05 compared with SB-treated animals by 1-way ANOVA. (B) Western blot analysis with specific antibodies against HO-1 and reduced NADP dehydrogenase (quinone)-1 (Nqo1) in the kidney from AA and SS mice in steady state and exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 11% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA animals; #P < .05 compared with SB-treated animals by the 1-way ANOVA. (C) Western blot analysis with specific antibodies against VCAM-1 and ICAM-1, in the kidney from AA and SS mice exposed to hemolysate, treated with either vehicle or CORM-401. GAPDH is used as protein loading control; 75 μg/μL of protein loaded on an 11% T, 2.5% C polyacrylamide gel. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle treated animals by the 1-way ANOVA.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal