Figure 5.
CORM-401 prevents hemolysate-induced lung damage and counteracts pulmonary inflammatory vasculopathy in humanized SCD (SS) mice. (A) Representative micro picture of hematoxylin and eosin–stained sections and Perls-stained sections of the lung at 200× magnification from SCD (SS) mice exposed to sterile buffer (SB) or hemolysate (AA hemolysate in AA recipient and SS hemolysate in SS recipient, respectively) and treated with either vehicle or CORM-401 (scale bar: 50 mm) (see also Table 3). (B) Western blot analysis with specific antibodies against phosphorylated (p)Nrf2 and Nrf2 in the lung from AA and SS mice infused with SB or hemolysate and treated with either vehicle or CORM-401. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is used as protein loading control. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots (DU: densitometric unit) is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA SB-treated animals; P < .05 compared with SS SB-treated animals by 1-way analysis of variance (ANOVA). (C) Western blot analysis with specific antibodies against HO-1 and TBXS in the lung from AA and SS mice infused with SB or hemolysate and treated with either vehicle or CORM-401. GAPDH is used as protein loading control. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown in the lower panel. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA mice treated with SB; #P < .05 compared with SS mice treated with SB by the 1-way ANOVA.

CORM-401 prevents hemolysate-induced lung damage and counteracts pulmonary inflammatory vasculopathy in humanized SCD (SS) mice. (A) Representative micro picture of hematoxylin and eosin–stained sections and Perls-stained sections of the lung at 200× magnification from SCD (SS) mice exposed to sterile buffer (SB) or hemolysate (AA hemolysate in AA recipient and SS hemolysate in SS recipient, respectively) and treated with either vehicle or CORM-401 (scale bar: 50 mm) (see also Table 3). (B) Western blot analysis with specific antibodies against phosphorylated (p)Nrf2 and Nrf2 in the lung from AA and SS mice infused with SB or hemolysate and treated with either vehicle or CORM-401. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is used as protein loading control. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots (DU: densitometric unit) is shown on the right. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA SB-treated animals; P < .05 compared with SS SB-treated animals by 1-way analysis of variance (ANOVA). (C) Western blot analysis with specific antibodies against HO-1 and TBXS in the lung from AA and SS mice infused with SB or hemolysate and treated with either vehicle or CORM-401. GAPDH is used as protein loading control. One representative gel from 5 with similar results is shown. Densitometric analysis of immunoblots is shown in the lower panel. Data represent mean ± SEM (n = 5); ^P < .05 compared with vehicle-treated animals; ∗P < .05 compared with AA mice treated with SB; #P < .05 compared with SS mice treated with SB by the 1-way ANOVA.

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