Effects of IFN-α on HSPCs from patients with MPN carrying JAK2-V617F and DNMT3A mutations. (A) Experimental setup for liquid cultures of single-cell FACS sorted HSPCs. (B) Growth efficiency of single-cell sorted HSPCs expressed as the percentages of wells in which colonies grew. (C) Genotyping of HSPC–derived colonies treated with IFN-α or vehicle. Stacked bars represent the percentages of colonies for each of the genotypes. Numbers above the bars indicate the numbers of colonies analyzed. (D) Relative change (%) in the numbers of JAK2-V617F-positive colonies. ANOVA with subsequent Sidak (D) posttest were used. FACS, fluorescence-activated cell sorting.