KO of GATA2AS increased human primary CD34⁺ cell differentiation. (A) Schematic of human CD34⁺ cell differentiation. Cells were infected with lentivirus containing shRNA on day 5 and transduction efficiency was 21%. (B) KD of GATA2AS by shRNA-1 and -2 in human CD34⁺ cells detected on days 6 and 10 by RT-qPCR. (C) Flow cytometry analysis of CD235a and CD71 expression of GATA2AS KD and control human CD34⁺ cells during differentiation. Percentage of cells for each quadrant is indicated. (D) MFI of CD235a and CD71 during erythroid differentiation. (E) Heat map of 5 groups of expression patterns for subsets of genes in GATA2AS KO and KD. (F) Expression curves for the 5 groups of genes shown in panel E. (G) GO term analysis of shared DEGs between GATA2AS KD CD34⁺ cells and KO HUDEP2 cells. (H) Examples of shared DEGs between GATA2AS KD CD34⁺ cells and KO HUDEP2 cells.