KO of GATA2AS affects chromatin accessibility on GATA2 and GATA1 switching sites. (A) MA plot showing differential ATAC-seq accessibility sites upon KO of GATA2AS in undifferentiated HUDEP2 cells. (B) Distribution of differentially accessible sites losing accessibility (left, down) or gaining accessibility (right, up) in the genome upon GATA2AS loss. (C) Heat maps of ATAC-seq signal on the 2 sets (with or without LRF binding) of GATA2AS binding peaks. (D) Motifs enriched in up and down differentially accessible sites were analyzed with HOMER. GATA1 and GATA2 motifs were enriched in both up and down differentially accessible sites. q-value for all motifs is <0.0001. (E) Heat maps showing GATA2 and GATA1 ChIP-seq signal of erythroid progenitor (Eprog) and precursor (Eprec) cells on sites that were up and down differentially accessible sites in GATA2AS KO cells. GATA1 binding on upregulated differentially accessible sites (blue trace) or downregulated sites (green trace) in upper graphs.