KO of GATA2AS in HUDEP2 cells decreased KLF1 and LRF binding genome wide. (A,C) MA plot of differential binding of KLF1(A) or LRF (C) in undifferentiated GATA2AS KO HUDEP2 cells compared with controls. (B,D) Genomic distribution of differential KLF1 (B) or LRF (D) binding sites. (E) Read density plot of KLF1 (left) and LRF (right) on binding sites after GATA2AS KO compared with control cells. (F) Venn diagram showing KLF1 or LRF diffbinds that are also GATA2AS DEGs at day 0 and their overlap. (G) Top panels show gene ontology (GO) terms enriched in KLF1 or LFR diffbinds that are also GATA2AS DEGs at day 0. Lower panels show GO terms for erythroid fingerprint genes in these groups. (H-I) Genome browser views of representative upregulated (HSPA9) and downregulated (EIF2A) genes after GATA2AS KO HUDEP2 cells. GATA2AS ChIRP-seq, RNA-seq, H3K27ac ChIP-seq, LRF or KLF1 ChIP-seq signal in KO control (KO_ctrl) and GATA2AS KO (KO_GATA2AS) are indicated.