Gene expression changes in HUDEP2 GATA2AS KO cells are consistent with phenotypic changes. (A) RNA-seq of GATA2AS KO and control HUDEP2 cells on day 0 of differentiation. MA plots of DEGs, the number of upregulated and downregulated genes, representative erythroid genes, and a twofold change threshold are indicated. (B) Venn diagram shows overlapping DEGs between different time points during differentiation for control and GATA2AS KO HUDEP2 cells. The number of overlapped DEGs is indicated. (C) Gene ontology (GO) analysis of differentially expressed genes and pathways associated with cell proliferation and erythroid differentiation are shown. (D) Differential gene expression compared with control is shown for select DEGs in HUDEP2 cells after loss of GATA2AS, including α-globins HBA1 and HBA2, adult HBB and HBD, fetal HBG1 and HBG2, and GATA1/2. (E) Heat map shows log2 fold change for DEGs in panel D at different days of differentiation of GATA2AS KO and control cells with P values superimposed. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.