Increased activation and EETosis in LNK(TT) cells. (A) p-STAT5 level was analyzed on day 18 in CD45⁺CD11b+Siglec8+ LNK(TT) and LNK(CC) cells (n = 3). Unpaired t test. (B) Surface CD11b level on day 18 in LNK(TT) and LNK(CC) cells (n = 3). (C) LNK(TT) and LNK(CC) eosinophils were stimulated with PAF for 4 hours. Representative images of EETosis. Green, MBP; red, citrullinated histone (H3Cit); blue, DAPI. Eosinophil extracellular trap (EET) numbers were normalized to MBP positive cell numbers; 2-way analysis of variance. Scale bar, 50 μm. Data are expressed as mean ± SEM.