Figure 1.
Characteristics of eosinophils and increased EETs in LNK deficiency. (A) Eosinophil counts or percentage in CD45+ cells in chow-fed and WTD mice. (B) Eosinophil activation marker (CD11b and CD69) expression in eosinophils from peripheral blood as assessed by flow cytometry. (C) Phospho-stat5 expression at basal and IL-5–stimulated conditions in WT and Lnk−/− BM-derived eosinophils assessed by flow cytometry. (D) Representative images and quantification of EETs (H3Cit+, red and MBP, green) at basal and stimulated conditions in WT and Lnk−/− BM-derived eosinophils. Scale bar, 100 μm. IgG, immunoglobulin G.

Characteristics of eosinophils and increased EETs in LNK deficiency. (A) Eosinophil counts or percentage in CD45+ cells in chow-fed and WTD mice. (B) Eosinophil activation marker (CD11b and CD69) expression in eosinophils from peripheral blood as assessed by flow cytometry. (C) Phospho-stat5 expression at basal and IL-5–stimulated conditions in WT and Lnk−/− BM-derived eosinophils assessed by flow cytometry. (D) Representative images and quantification of EETs (H3Cit+, red and MBP, green) at basal and stimulated conditions in WT and Lnk−/− BM-derived eosinophils. Scale bar, 100 μm. IgG, immunoglobulin G.

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