CD8 T cells from the BM egress into the PB during stem cell mobilization. (A) T cells were harvested from the BM and spleen of MM-bearing mice (CD45.1) and transferred to naïve B6 mice (CD45.2). Recipient mice were euthanized 1 week after adoptive transfer for analysis. The absolute count of total and CD44⁺ memory CD8 T cells (CD45.1) in the BM, blood, and spleen (n = 7 per group from 2 independent experiments). (B) BM T cells from MM-bearing mice (CD45.1) were harvested and transferred to naïve B6 mice (CD45.2). B6 mice were treated with G-CSF⁺AMD3100 or PBS 1 week after T-cell transfer and then euthanized to measure the absolute counts of transferred T cells (CD45.1) in the BM, blood, and spleen. Donor T cells are shown as fold change compared with the number in the nonmobilized group (n = 7 per group from 2 independent experiments). (C) Female MataHari HY-specific TCR transgenic CD8 T cells (CD45.1) were transferred into male (HY⁺) VkMYC (Vk28158)-bearing female RAG2/IL2rg KO mice, followed by G-CSF⁺AMD3100 or control vehicle injection. Total number of CD45.1⁺ MataHari CD8 T cells is shown (n = 4 per group from 2 independent experiments). Data represents mean ± SEM. Welch t test or Mann-Whitney test for 2 samples comparison. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001.