Effects of RAC2 mutations on cellular localization and morphology. COS-7 cells cotransfected with GFP (green) and various RAC2 mutants were stained for F-actin (red) and RAC2 (white), nuclei were counterstained with 4′,6-diamidino-2-phenylindole (blue). Representative merged images are shown. Scale bar, 10 μm. Yellow arrows, PM ruffling; yellow arrowheads, macropinosomes.