Inhibition of thrombosis by blockade of plasma-driven translation in murine platelets. (A) Time to cessation of respiration in mice treated as indicated, after bolus administration of 900 U/kg thrombin to induce pulmonary thromboembolism; n = 6; P < .01. (B) Naïve mice were transfused with 1 × 10⁸ washed platelets from vehicle- or puromycin-treated mice, and subject to thrombin administration and monitoring as in panel A. Time to cessation of respiration is shown. Veh, vehicle; puro, puromycin. n = 3; P < .03. (C) Representative images of hematoxylin/eosin-stained lung sections from mice from panel B, perfused with saline immediately after the experimental end point, before lung resection. Photographs of the resected lungs before sectioning are shown below. Lungs from mice receiving vehicle-treated platelets appeared blood-filled, indicating thrombosis preventing saline perfusion, whereas lungs from mice receiving puromycin-treated platelets appeared to contain less blood, indicating reduced thrombosis. (D) Carotid arteries in WT mice treated with vehicle or puromycin as indicated were injured with 7.5% FeCl₃. Flow rates through the carotid artery downstream of the injury site were monitored with a Doppler probe. Time to cessation of blood flow is shown. Two representative tracings showing blood flow before, during, and after injury per treatment are shown to the right; n = 9 vehicle, n = 6 puromycin; P < .005. (E) WT untreated (naïve) mice transfused with 1 × 10⁸ washed platelets freshly isolated from vehicle- or puromycin-treated mice were subject to FeCl₃ carotid arterial injury as in panel D; n = 5 naïve + vehicle platelets, n = 6 naïve + puromycin platelets; P < .006. Vehicle or vehicle platelet-infused, green closed circles and solid green lines; puromycin or puro platelet-infused, red open circles and dotted red lines.