Figure 7.
![FGL1 is a BMP antagonist. Relative expression of Hamp (A), Id1 (B), Smad7 (C) mRNA expression in mouse primary hepatocytes treated with BMP ligands (10 ng/mL) and human Fc immunoglobulin G2 (IgG2; control [CTRL], 10 μg/mL) or Fc-FGL1 (mouse FGL1, 10 μg/mL) for 15 hours relative to untreated cells in serum-free conditions. Data shown are mean ± SEM of 3 independent experiments and were compared for each BMP between FGL1-treated cells and Fc-treated cells using the Student t test. ∗∗∗∗P < .0001. (D) Western blotting of Hep3B cells treated for 6 hours with BMP6 (10 ng/mL), ERFE (1 μg/mL), or FGL1 (10 μg/mL) for P-SMAD5, SMAD5, and GAPDH. (E) Western blotting of pull-down assay of BMP6 and FGL1 (FL, glob, and Nter) or human Fc IgG2.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/143/13/10.1182_blood.2023022724/1/blood_bld-2023-022724-gr7.jpeg?Expires=1749646265&Signature=cavTE7x~H~FoxKpDXF0-ZIO~7GL62LTcmuXF4bDscXuav9TOZbFW4fihw3BwMJhMX9OdnO2nYh-FNGK4QecsdjN8eu1L7dk9OFNgrcL~M9y9jE8zbpL4yD8GL20pPCoOHypvbvkgFqanOv2JTISrJICf9kmD3H9ywkAUJr1XLUTtZpy5qGx5vMBtTFbMMOnT4~aXII3xyvT-19lFMx1yCpW9AEsO277rfIr4CX6-5aSs7H~VwBnGxKJOqUsbqLSn0HctmTK3vIAFIvj0vus~R1GhdF2bz6Ct-7NL4yCUr-GHmyeFBVdvkhpktZ80KkQOhg1AuarGH~hIxxblK7E1Jg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FGL1 is a BMP antagonist. Relative expression of Hamp (A), Id1 (B), Smad7 (C) mRNA expression in mouse primary hepatocytes treated with BMP ligands (10 ng/mL) and human Fc immunoglobulin G2 (IgG2; control [CTRL], 10 μg/mL) or Fc-FGL1 (mouse FGL1, 10 μg/mL) for 15 hours relative to untreated cells in serum-free conditions. Data shown are mean ± SEM of 3 independent experiments and were compared for each BMP between FGL1-treated cells and Fc-treated cells using the Student t test. ∗∗∗∗P < .0001. (D) Western blotting of Hep3B cells treated for 6 hours with BMP6 (10 ng/mL), ERFE (1 μg/mL), or FGL1 (10 μg/mL) for P-SMAD5, SMAD5, and GAPDH. (E) Western blotting of pull-down assay of BMP6 and FGL1 (FL, glob, and Nter) or human Fc IgG2.
