Figure 4.
FGL1 is a suppressor of hepcidin in vitro and in vivo.HAMP mRNA expression in Hep3B and HepG2 cells cultured in serum-free medium in response to BMP6 (25 ng/mL, 6 hours) (A) or BMP6 + recombinant mouse FGL1 (1-10 μg/mL) (B). (C) ID1 expression in hepatoma cell lines treated with BMP6 and FGL1. Hepatic Hamp mRNA expression (D), serum hepcidin concentration (E), and liver Id1 (F) and Smad7 (G) mRNA expression, serum (H) and liver iron (I) concentration in mice treated for 6 hours with saline, Fc, or recombinant mouse FGL1 (10 mg/kg; n = 5). Data shown are mean ± SEM of 3 independent experiments (A-C) and were compared with untreated cells using the Student t test (A) or 1-way ANOVA (B-I) and corrected for multiple comparisons using the Holm-Šidák method. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.

FGL1 is a suppressor of hepcidin in vitro and in vivo.HAMP mRNA expression in Hep3B and HepG2 cells cultured in serum-free medium in response to BMP6 (25 ng/mL, 6 hours) (A) or BMP6 + recombinant mouse FGL1 (1-10 μg/mL) (B). (C) ID1 expression in hepatoma cell lines treated with BMP6 and FGL1. Hepatic Hamp mRNA expression (D), serum hepcidin concentration (E), and liver Id1 (F) and Smad7 (G) mRNA expression, serum (H) and liver iron (I) concentration in mice treated for 6 hours with saline, Fc, or recombinant mouse FGL1 (10 mg/kg; n = 5). Data shown are mean ± SEM of 3 independent experiments (A-C) and were compared with untreated cells using the Student t test (A) or 1-way ANOVA (B-I) and corrected for multiple comparisons using the Holm-Šidák method. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.

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