MIR155HGΔexon3 T cells display markedly reduced proliferation and Th1 population in GVHD target organ. Xenogeneic GVHD transplants were performed as before, and recipient NSG mice were euthanized at either day 17 or day 35 after transplantation to obtain single-cell suspension from GVHD target organ liver for flow cytometric analyses. N = 4 mice for each time point, data combined from 2 independent donors. All data are shown as absolute counts (cells per μL). (A) CD3+ T cells in liver on day 17 (left) and day 35 (right) after transplantation. (B) CD4+ and CD8+ T-cell subsets. (C) Percentage Ki67 expression on CD4+ T cells. (D) Absolute numbers of Ki67+ CD4+ and Ki67+ CD8+ T cells at day 17 (left) and day 35 (right) after transplantation. (E) Representative day 17 contour plots from panel C. (F) Proportion of Th1, Th17, and Treg CD4+ T-cell subtypes on day 35 after transplantation. (G) IFN-γ expressing CD4+ T cells on day 35 after transplantation.
