Figure 3.
Proliferative and transcriptional effects of SGF29 loss in AML. Proliferation assays in SGF29 wild-type or CRISPR knockout AML cell lines U937 (A) or MOLM13 (B) are shown over 10 days as indicated on the x-axis (n = 3). (C) Retention of the CellTrace Far Red dye in U937 cells transduced with an SGF29 sgRNA (bottom panel) compared with NTC (top panel), measured using flow cytometry over time (representative experiment from n = 3 is shown). (D) Schematic for differentiation assessment via phagocytosis of fluorescently labeled E coli bioparticles. (E) Histograms of U937 cells transduced with an NTC (gray) or an SGF29 sgRNA (blue) showing the fluorescence intensity of phagocytosed pHrodo Red E coli bioparticles (n = 3). (F) Histograms of U937 cells transduced with an NTC (gray) or an SGF29 sgRNA (blue) showing the normalized fluorescence intensity of CD11b on the x-axis (n = 3). (G-H) Volcano plot of genes differentially changed in SGF29 deleted, compared with NTC cells in U937 (G) or MOLM13 (H) is shown with key leukemia-associated and differentiation-associated genes marked. (I) Proliferation assays of SGF29 wild-type or deficient UB3 cells with or without retroviral overexpression of HOXA9-MEIS1 are shown over a period of 10 days (n = 3). (J) Select categories of gene sets showing significantly lower enrichment in SGF29 deleted MOLM13 cells compared with wild-type counterparts using gene set enrichment analysis (GSEA) are shown in the bubble plot. Normalized enrichment score (NES) is plotted on the x-axis, and bubbles are colored by FDR-q value and sized by gene set sizes. (K) Select GSEA plots with NES and FDR-q values are shown for gene sets associated with myeloid development, MYC-targets, and LSCs. (L-M) Kaplan-Meier survival curves for NTC vs SGF29 knockout in U937 (L) and MOLM13 (M) AML cell lines injected mice are shown. Five mice per group were injected with cells expressing NTC or SGF29 sgRNA.

Proliferative and transcriptional effects of SGF29 loss in AML. Proliferation assays in SGF29 wild-type or CRISPR knockout AML cell lines U937 (A) or MOLM13 (B) are shown over 10 days as indicated on the x-axis (n = 3). (C) Retention of the CellTrace Far Red dye in U937 cells transduced with an SGF29 sgRNA (bottom panel) compared with NTC (top panel), measured using flow cytometry over time (representative experiment from n = 3 is shown). (D) Schematic for differentiation assessment via phagocytosis of fluorescently labeled E coli bioparticles. (E) Histograms of U937 cells transduced with an NTC (gray) or an SGF29 sgRNA (blue) showing the fluorescence intensity of phagocytosed pHrodo Red E coli bioparticles (n = 3). (F) Histograms of U937 cells transduced with an NTC (gray) or an SGF29 sgRNA (blue) showing the normalized fluorescence intensity of CD11b on the x-axis (n = 3). (G-H) Volcano plot of genes differentially changed in SGF29 deleted, compared with NTC cells in U937 (G) or MOLM13 (H) is shown with key leukemia-associated and differentiation-associated genes marked. (I) Proliferation assays of SGF29 wild-type or deficient UB3 cells with or without retroviral overexpression of HOXA9-MEIS1 are shown over a period of 10 days (n = 3). (J) Select categories of gene sets showing significantly lower enrichment in SGF29 deleted MOLM13 cells compared with wild-type counterparts using gene set enrichment analysis (GSEA) are shown in the bubble plot. Normalized enrichment score (NES) is plotted on the x-axis, and bubbles are colored by FDR-q value and sized by gene set sizes. (K) Select GSEA plots with NES and FDR-q values are shown for gene sets associated with myeloid development, MYC-targets, and LSCs. (L-M) Kaplan-Meier survival curves for NTC vs SGF29 knockout in U937 (L) and MOLM13 (M) AML cell lines injected mice are shown. Five mice per group were injected with cells expressing NTC or SGF29 sgRNA.

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