Gprc5c expression levels regulate the development of leukemia. (A) Overall survival according to GPRC5C expression in the Leucegene cohort. High expression levels of GPRC5C (GPRC5C high: ≥75th percentile, red curve) compared with low expression levels (GPRC5C low: <75th percentile, blue curve) are displayed by Kaplan-Meier curves. The P-value was obtained using the log-rank test for the comparison of survival curves. (B) Blue box: schematic representation of the experimental design to overexpress and assess the impact of increased Gprc5c levels in MLL-AF9. Red box: schematic representation of the experimental design to evaluate the effect of the loss of Gprc5c in MLL-AF9. (C) Survival curve depicting AML penetrance upon the gain of Gprc5c in MLL-AF9; n = 9. (D) Survival curve depicting AML penetrance upon loss of Gprc5c in MLL-AF9. Combination of 2 independent experiments; n = 5 to 7. (E) Limiting dilution assays for MLL-AF9-WT and MLL-AF9-Gprc5c-KO. Combination of 2 independent experiments. All represented by mean ± standard deviation. (C-D) Mantel-Cox test, ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P <.0001. For all experiments, at least 2 independent experiments were performed. ns, not significant.