Loss of Tet2 exacerbates MSU crystal-induced inflammatory phenotype in murine models. (A) Serum cytokine array of Tet2-deficient (Tet2 KO, n = 5) vs WT (n = 5) mice after 6 hours of treatment with PBS (left plot) and MSU crystals (right plot). Blue dots highlight cytokines with P < .05 using 2-sample Student t test; red dots highlight cytokines with adjustment of false discovery rate (FDR < 0.1). (B) Flow cytometry analysis of peripheral blood and peritoneal fluid from WT and Tet2 KO mice after 6 hours of treatment with PBS and MSU crystals. Two-sample Student t test was used to compare the fraction of CD11b⁺ cells between MSU-treated WT and Tet2 KO animals. (C) Paw edema elicited by subcutaneous injection of MSU crystals into the foot pads of WT (n = 10) and Tet2 KO (n = 10) mice. P values were calculated using 2-way analysis of variance. (D) Representative hematoxylin and eosin stained paw cross sections of WT and Tet2 KO mice treated with PBS and MSU crystals shows increased cellular infiltrate (40× magnification).