Effects of ferroportin inhibition on MPN phenotype in mice with PV. (A) Schematic drawing of the experimental setup for the BM transplantations and treatment with ferroportin inhibitor vamifeport and the hepcidin mimetic PR73. (B) Time course of body weight (n = 8 mice per group). (C) Complete blood counts of recipient mice (n = 8 mice per group) are shown at the indicated times. (D) Analysis of the spleen and liver at terminal workup. (Top) Spleen and liver weight, (bottom) iron concentration in the spleen and liver. (E) Iron parameters and regulatory cytokines at terminal workup. Note that logarithmic scales are used for iron regulatory cytokines (Epo, erythroferrone, and hepcidin). All data are presented as mean ± standard error of the mean. One- and two-way ANOVA with subsequent Dunnett posttest were used. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.