Analysis of leukemias developed in MOL4070A-infected Utx^Δ/Δand Utx^Δ/Utymice. (A) Experimental procedure of retroviral insertional mutagenesis (top). Neonates were infected with MOL4070A, and leukemic mice were analyzed for disease phenotypes and virus integration sites (middle); survival curves are shown. Representative fluorescence-activated cell sorting results of leukemia cells (bottom), including acute myeloid leukemia (AML), T-lineage acute lymphoblastic leukemia (T-ALL), and B-lineage acute lymphoblastic leukemia (B-ALL). (B) Experimental procedure (top). c-kit⁺ cells were transduced with Sox4-IRES-EGFP EV and EGFP⁺ cells were subjected to serial replating and BMT assays and the colony-forming ability of Utx^+/++Sox4 and Utx^Δ/Δ+Sox4 cells was observed (second row). Colony numbers at rounds 1 to 4 (R1 to R4) of replating, starting at 1 × 10⁴ cells, and representative micrographs of colonies at R4 are shown. *P < .05; ***P < .001. Survival curves of transplant recipients (third row). Representative FACS results of AML cells developed in Utx^+/++Sox4 transplant recipients (bottom left). GSEA plots of hedgehog signaling (bottom right). The plots are shown with normalized enrichment score (NES) and false discovery rate (FDR).