NK cells expand in patients treated with DLI and ML NK cell infusion. Peripheral blood and bone marrow samples from patients were analyzed by CyTOF. (A) Representative viSNE demonstrating FlowSOM-gated lymphocyte populations (CD34⁻CD45⁺CD14⁻). (B) Heat map of each marker used to annotate FlowSOM-gated lymphocytes in panel A (C) Density map of lymphocytes at the indicated time points from a representative patient’s peripheral blood mononuclear cells. Inset numbers depict the percentage of each population within the indicated cluster. (D) Total number of cells of each lymphocyte population quantitated by CyTOF across the indicated time points in the peripheral blood of patients. (E-F) Percentage of each lymphocyte population quantitated by CyTOF in the peripheral blood (E) and bone marrow (F). CyTOF data were available for patients P-ML002, P-ML003, P-ML005, P-ML007, P-ML008, P-ML009, and P-ML011​. (D-F) Data are expressed as the mean ± standard error. CyTOF, cytometry by time of flight; viSNE, visual stochastic neighbor embedding.